Isolation and Identification of Calystegines in Root Cultures of four Physalis Species

Calystegines were isolated from the root cultures of Physalis divaricata, P. pubescens, P. philadelphia,and P. philadelphia (solanaceae). Calystegines of P. divaricata were identified as calystegine A3, A5, B1 and B2, with concentrations of 6.99, 4.41, 8.52, and 14.7 ?g/g in fresh mass, respectively. Root cultures of P.pubescens contain calystegine A3, B1 and B2, P. philadelphia only contains calystegine B2 and P. philadelphia root cultures solely consist of calystegine A3. Isolation and identification of calystegines have been achieved by ion exchange column chromatography and gas chromatography, using authentic samples.